A comparison of brain heart infusion blood agar sterilized by filtration and heat on the growth of Neisseria gonorrhoeae.
نویسندگان
چکیده
The growth of Neisseria gonorrhoeae on brain heart infusion blood agar in which the base was sterilized by filtration has been compared with growth on the same medium sterilized by heat. Colonies were larger on the unheated medium, and autoclaving at 115 degrees C of 121 degrees C for 15 minutes was accompanied by a progressive decrease in colony size. Viable counts on the three media showed no difference in end points. Colonies on the unheated medium were usually large enough to be easily recognizable after overnight incubation.
منابع مشابه
A study on the frequency of vaginal species of Mycoplasma genitalium, Gardnerella vaginalis and Neisseria gonorrhoeae among pregnant women by PCR technique
Bacterial vaginosis or non-specific vaginitis describes the disease caused by a change in the normal Flora of the vagina, which leads to the elimination of Lactobacilli, generating hydrogen peroxide and excess growth of bacteria, particularly anaerobic bacteria. This disease is the most prevalent infection of the female genital tract, and the rate of frequency of anaerobic bacteria, specificall...
متن کاملCombined selective descriptive medium for the culture of Neisseria gonorrhoeae.
A selective identification medium for Neisseria gonorrhoeae was devised by incorporating antibiotics and a mixture of sugars into a yeast extract agar. The results were compared with those using a standard combined selective/non-selective blood agar medium. Recovery rates of N gonorrhoeae from specimens from 208 patients cultured on both media were comparable, but the selective descriptive medi...
متن کاملL form of Neisseria gonorrhoeae.
Roberts, Richard B. (Walter Reed Army Institute of Research, Washington, D.C.). L form of Neisseria gonorrhoeae. J. Bacteriol. 92:1609-1614. 1966.-L forms were produced by the penicillin gradient plate technique from a recently isolated strain of Neisseria gonorrhoeae. To date, these L forms have had 30 serial passages on medium containing penicillin. Stabilized L forms developed on penicillin-...
متن کاملBulk growth of Neisseria gonorrhoeae type 1 in a biphasic system.
A biphasic system for the bulk growth of Neisseria gonorrhoeae is described. It appears to combine the convenience of liquid media with the growth qualities of solid media. By using the agar in dialysis tubes rather than as slabs the surface-to-volume ratio was improved and harvesting made easier. The system is also useful in isolating gonococci from blood and joint fluids in patients with diss...
متن کاملEffect of sodium polyanetholesulfonate and gelatin on the recovery of Gardnerella vaginalis from blood culture media.
Sodium polyanetholesulfonate (SPS) is used as a routine supplement to blood culture media to enhance recovery of microorganisms, but it inhibits the growth of Peptostreptococcus anaerobius, Neisseria meningitidis, Neisseria gonorrhoeae, and Streptobacillus moniliformis. Comparative clinical blood culture studies at the University of Colorado Hospital suggested that SPS also inhibits the growth ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of clinical pathology
دوره 29 12 شماره
صفحات -
تاریخ انتشار 1976